Allododecaploid yeasts: synthetic hybrids of six species

David Peris, Ryan V. Moriarty, William G. Alexander, Russell L. Wrobel, Chris Todd Hittinger

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Jun 06, 2019

Received Date: 27th May 19

Polyploidy generates diversity by increasing the number of copies of each chromosome 1 . Many plants, animals, fungi, and other eukaryotes are ancient or recent polyploids, including some of the best-known evolutionary radiations, crops, and industrial organisms 2,3. Polyploidy facilitates differentiation and adaptation to new environments, but the tools to test its limits are lacking. Here we develop an iterative Hybrid Production (iHyPr) method to produce allododecaploid (base ploidy 12n) hybrids of six yeast species, increase chromosomal instability, and improve strain performance during adaptive laboratory evolution. Polyploidization promotes genome instability and increases the mutation rate, which can facilitate short-term adaptation of organisms to new environments 4 or lead to tumorigenesis in some cancer types 5. This new approach for making higher-order synthetic hybrids will enable basic research on polyploidy, cancer, and chromosome biology, as well as natural diversity-driven approaches 6 to applied to research on biofuels, bioproducts, and synthetic biology.

Read in full at bioRxiv.

This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

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Nature Communications

Nature Research, Springer Nature