CDC20B is required for deuterosome-mediated centriole production in multiciliated cells

Diego R. Revinski, Laure-Emmanuelle Zaragosi, Camille Boutin, Sandra Ruiz-Garcia, Marie Deprez, Olivier Rosnet, Virginie Thomé, Olivier Mercey, Agnès Paquet, Nicolas Pons, Brice Marcet, Laurent Kodjabachian, Pascal Barbry

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Received: 6th November 17

Multiciliated cells (MCCs) harbour dozens to hundreds of motile cilia, which beat in a synchronized and directional manner, thus generating hydrodynamic forces important in animal physiology1, 2. In vertebrates, MCC differentiation critically depends on the synthesis and release of numerous centrioles by specialized structures called deuterosomes1-5. Little is known about the composition, organization and regulation of deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome-stage MCCs are characterized by the expression of many cell cycle-related genes. Among those, we further investigated the uncharacterized vertebrate-specific cell division cycle 20B (CDC20B) gene. We show that the CDC20B protein associates to the deuterosome, and is required for the production of centrioles and cilia in mouse and Xenopus MCCs. In Xenopus, centrioles and cilia were efficiently rescued in absence of CDC20B by over-expression of the protease Separase, linking CDC20B function to centriole release from deuterosomes, in analogy to centriole disengagement in mitotic cells. This work reveals the shaping of a new biological function, deuterosome-mediated centriole production in vertebrate MCCs, by adaptation between ancestral and recently evolved cell cycle-related molecules.

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This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

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