An unconventional myosin, myosin 1d regulates Kupffer’s vesicle morphogenesis and laterality

Manush Saydmohammed, Hisato Yagi, Michael Calderon, Madeline J. Clark, Timothy Feinstein, Ming Sun, Donna B. Stolz, Simon C. Watkins, Jeffrey D. Amack, Cecilia W. Lo, Michael Tsang

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Received: 19th February 18

Establishing left-right (LR) asymmetry is a fundamental process essential for arrangement of visceral organs during development. In vertebrates, motile cilia driven fluid flow in the left-right organizer (LRO) is essential for initiating symmetry breaking event1-3. Without a definite LRO structure in invertebrates, LR asymmetry is initiated at a cellular level by actin-myosin driven chirality4, 5. In Drosophila, myosin1D drives tissue-specific chirality in hind-gut looping6, 7. Here, we report that myosin 1d (myo1d) is essential for establishing LR asymmetry in zebrafish. Using super-resolution microscopy, we show that the zebrafish LRO, Kupffer's vesicle (KV), fails to form a spherical lumen and unidirectional flow in the absence of myo1d. This process requires directed vacuolar trafficking in KV epithelial cells. Interestingly, the vacuole transporting function of zebrafish Myo1d can be substituted by myosin1C derived from an ancient eukaryote, Acanthamoeba castellanii, where it regulates the transport of contractile vacuoles. Our findings reveal a role for an unconventional myosin in vacuole trafficking, lumen formation and determining laterality.

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This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

Nature Communications

Nature Research, Springer Nature