A spike-timing-dependent plasticity rule for single, clustered and distributed dendritic spines

Sabrina Tazerart, Diana E. Mitchell, Soledad Miranda-Rottmann , and Roberto Araya

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Received Date: 16th May 19

The structural organization of excitatory inputs that supports spike-timing-dependent plasticity (STDP) remains unknown. Here we performed a spine STDP protocol using two-photon glutamate uncaging (pre) paired with postsynaptic spikes (post). We found that pre-post pairings that trigger LTP (t-LTP) produce shrinkage of the activated spine neck and increase in synaptic strength; and post-pre pairings that trigger LTD (t-LTD) decrease synaptic strength without affecting spine shape. Furthermore, we tested whether this rule could be affected by the activation of two clustered or distributed spines. We show that the induction of t-LTP in clustered spines (<5 μm apart) enhances LTP through a mechanism dependent on spine calcium accumulation and actin polymerization-dependent neck shrinkage, whereas t-LTD was disrupted by the activation of clustered spines but recovered when separated by >40 μm. These results indicate that synaptic cooperativity disrupts t-LTD and extends the temporal window for the induction of t-LTP, leading to STDP only encompassing LTP.

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This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

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