Dynamic Sex Chromosome Expression in Drosophila Male Germ Cells

Sharvani Mahadevaraju, et al.

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Received Date: 11th April 20

Sharvani Mahadevaraju, Justin M. Fear, Miriam Akeju, Brian J. Galletta, Mara MLS. Pinheiro, Camila C. Avelino, Diogo C. Cabral-de-Mello, Katie Conlon, Stafania Dell’Orso, Zelalem Demere, Kush Mansuria, Carolina A. Mendonça, Octavio M. Palacios-Gimenez, Eli Ross, Max Savery, Kevin Yu, Harold E. Smith, Vittorio Sartorelli, Nasser M. Rusan, Maria D. Vibranovski, Erika Matunis, Brian Oliver

Sex chromosome gene content and expression is unusual. In many organisms the X and Y chromosomes are inactivated in spermatocytes, possibly as a defense mechanism against insertions into unpaired chromatin. In addition to current sex chromosomes, Drosophila has a small gene-poor X-chromosome relic (4th) that re-acquired autosomal status. Using single cell RNA-Seq, we demonstrate that the single X and pair of 4th chromosomes are specifically inactivated in primary spermatocytes. In contrast, genes on the single Y chromosome become maximally active in primary spermatocytes. Reduced X steady-state transcript levels are due to failed activation of RNA-Polymerase-II by phosphorylation of Serine 2 and 5.

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This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

Nature Communications

Nature Research, Springer Nature