Stepwise evolution and convergent recombination underlie the global dissemination of carbapenemase-producing Escherichia coli

Rafael PATIÑO-NAVARRETE, Isabelle ROSINSKI-CHUPIN, Nicolas CABANEL, Lauraine GAUTHIER, Julie TAKISSIAN, Jean-Yves MADEC, Monzer HAMZE, Remy A. BONNIN, Thierry NAAS and Philippe GLASER

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Nov 06, 2018
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Received Date: 14th October 18

Carbapenem-resistant Enterobacteriaceae are considered by WHO as “critical” priority pathogens for which novel antibiotics are urgently needed. The dissemination of carbapenemase-producing Escherichia coli(CP-Ec) in the community is a major public health concern. However, the global molecular epidemiology of CP-Ec isolates, as well as the genetic bases for the emergence and global dissemination of specific lineages, remain largely unknown. Here, by combining a thorough genomic and evolutionary analysis of Ec ST410 isolates with a broad analysis of 12,398 E. coli and Shigella genomes, we showed that the fixation of carbapenemase genes depends largely on a combination of mutations in ftsI encoding the penicillin binding protein 3 and in the porin genes ompCand ompF. Mutated ftsI genes and a specific ompC allele spread across the species by recombination. Those mutations were in most cases selected prior to carbapenemase gene acquisition. The selection of CP-Ec lineages able to disseminate is more complex than the mere acquisition of carbapenemase genes and might be largely triggered by b-lactams other than carbapenems.

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This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

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