Stepwise evolution and convergent recombination underlie the global dissemination of carbapenemase-producing Escherichia coli

Rafael PATIÑO-NAVARRETE, Isabelle ROSINSKI-CHUPIN, Nicolas CABANEL, Lauraine GAUTHIER, Julie TAKISSIAN, Jean-Yves MADEC, Monzer HAMZE, Remy A. BONNIN, Thierry NAAS and Philippe GLASER

Thumb 65bb0659497b85bae0759dc2a6b4b5db 400x400
Nov 06, 2018

Received Date: 14th October 18

Carbapenem-resistant Enterobacteriaceae are considered by WHO as “critical” priority pathogens for which novel antibiotics are urgently needed. The dissemination of carbapenemase-producing Escherichia coli(CP-Ec) in the community is a major public health concern. However, the global molecular epidemiology of CP-Ec isolates, as well as the genetic bases for the emergence and global dissemination of specific lineages, remain largely unknown. Here, by combining a thorough genomic and evolutionary analysis of Ec ST410 isolates with a broad analysis of 12,398 E. coli and Shigella genomes, we showed that the fixation of carbapenemase genes depends largely on a combination of mutations in ftsI encoding the penicillin binding protein 3 and in the porin genes ompCand ompF. Mutated ftsI genes and a specific ompC allele spread across the species by recombination. Those mutations were in most cases selected prior to carbapenemase gene acquisition. The selection of CP-Ec lineages able to disseminate is more complex than the mere acquisition of carbapenemase genes and might be largely triggered by b-lactams other than carbapenems.

Read in full at bioRxiv.

This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

Medium 65bb0659497b85bae0759dc2a6b4b5db 400x400

Nature Communications

Nature Research, Springer Nature