Rapid single-wavelength lightsheet localization microscopy for clarified tissue

Li-An Chu, Chieh-Han Lu, Shun-Min Yang, Yen-Ting Liu, Yun-Chi Tsai, Shu-Wei Chang, Peilin Chen, Ting-Kuo Lee, Yeu-Kuang Hwu, Ann-Shyn Chiang, Bi-Chang Chen

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Jan 04, 2019
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Received Date: 12th December 18

To overcome the aberration encountered in super-resolution imaging of thick tissue, we used a single-wavelength Bessel lightsheet microscope optimized for refractive-index matching with clarified specimens. We resolved nanoscopic Dopaminergic neurons distribution across an intact Drosophila brain (3.64 x 107µm3) with high imaging speed (436 µm3/sec). Maneuvering the high throughput (one Drosophila brain per day), huge data can be accessed to provide solid statistical basis for neurobiology.

Read in full at bioRxiv.

This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

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Nature Communications

Nature Research, Springer Nature