YAP-independent mechanotransduction drives breast cancer progression

Joanna Y. Lee, Jessica Chang, Antonia A. Dominguez, Sungmin Nam, Julie Chang, Hongpyo Lee, Sushama Varma, Lei S. Qi, Robert B. West and Ovijit Chaudhuri

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Jan 22, 2019
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Received Date: 7th January 19

Increased tissue stiffness is a driver of breast cancer progression. The transcriptional regulator YAP is considered a universal mechanotransducer, based largely on 2D culture studies. However, the role of YAP during in vivo breast cancer remains unclear. Here, we find that mechanotransduction occurs independently of YAP in breast cancer patient samples and mechanically tunable 3D cultures. Mechanistically, the lack of YAP activity in 3D culture and in vivo is associated with the absence of stress fibers and an order of magnitude decrease in nuclear cross-sectional area relative to 2D culture. This work highlights the context-dependent role of YAP in mechanotransduction, and establishes that YAP does not mediate mechanotransduction in breast cancer.

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This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.


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