Highly durable crack sensor integrated with silicone rubber cantilever for measuring cardiac contractility in culture media

Dong-Su Kim, Yong Whan Choi, Yun-Jin Jeong, Jongsung Park, Nomin-Erdene Oyunbaatar, Eung-Sam Kim, Mansoo Choi, and Dong-Weon Lee

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Feb 14, 2019
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Received Date: 23rd January 19

We propose a novel cantilever device integrated with a PDMS-encapsulated crack sensor that can measure the contraction behavior of cardiomyocytes. The crack sensor was chemically bonded to a PDMS thin layer to form a sandwiched structure that operates stably in culture media. The reliability of the proposed crack sensor was also dramatically improved compared with previous crack sensors. After evaluating the stability of the crack sensor bonded with the PDMS layer, cardiomyocytes were cultured on the nano-patterned cantilever for real-time measurement of cardiac contractile forces. The highly sensitive crack sensor stably measured the cardiac contractility without changing its gauge factor for up to 26 days (>5 million heartbeats). In addition, changes in contractile force induced by drugs were measured using the crack sensor-integrated cantilever, and our experimental results were compared with those obtained via conventional electrophysiological methods to verify the feasibility of building a contraction-based drug-toxicity testing system.

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This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.


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