Young Genes are More Responsive to Environmental Stress than Ancient Genes in Budding Yeasts

Tyler W. Doughty, Iván Domenzain, Aaron Millan-Oropeza, Noemi Montini, Philip A. de Groot, Rui Pereira, Jens Nielsen, Céline Henry, Jean-Marc G. Daran, Verena Siewers & John P. Morrissey

Thumb 65bb0659497b85bae0759dc2a6b4b5db 400x400
Jun 10, 2019

Received Date: 28th May 19

Members of the subphylum Saccharomycotina (budding yeasts) are found in many of

Earth’s biomes, with some species, including Saccharomyces cerevisiae,

Kluyveromyces marxianus, and Yarrowia lipolytica, exhibiting potential for industrial

production of biochemicals. One complication for production is that ideal growth

conditions (e.g. temperature, pH, salinity) for these species may not match reaction

parameters for industrial processes, resulting in cellular stress and lower product

yields. This work exposed budding yeasts to common industrial stressors to assess

commonalities amongst stress-adaptation responses. The primary finding was that

each stress-response, for each yeast species, was enriched for expression changes

amongst genus and species-exclusive (young) genes compared to broadly

conserved (ancient) genes. Further, young genes exhibited lower expression, less

influence on viability, and accumulated nonsynonymous substitutions more frequently

than ancient genes. These findings demonstrate that young genes are enriched for

stress-responsiveness, may adapt rapidly, and therefore, may be important targets

for improving industrial stress tolerances.

Read in full at bioRxiv.

This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

Medium 65bb0659497b85bae0759dc2a6b4b5db 400x400

Nature Communications

Nature Research, Springer Nature