Young Genes are More Responsive to Environmental Stress than Ancient Genes in Budding Yeasts
Tyler W. Doughty, Iván Domenzain, Aaron Millan-Oropeza, Noemi Montini, Philip A. de Groot, Rui Pereira, Jens Nielsen, Céline Henry, Jean-Marc G. Daran, Verena Siewers & John P. Morrissey
Received Date: 28th May 19
Members of the subphylum Saccharomycotina (budding yeasts) are found in many of
Earth’s biomes, with some species, including Saccharomyces cerevisiae,
Kluyveromyces marxianus, and Yarrowia lipolytica, exhibiting potential for industrial
production of biochemicals. One complication for production is that ideal growth
conditions (e.g. temperature, pH, salinity) for these species may not match reaction
parameters for industrial processes, resulting in cellular stress and lower product
yields. This work exposed budding yeasts to common industrial stressors to assess
commonalities amongst stress-adaptation responses. The primary finding was that
each stress-response, for each yeast species, was enriched for expression changes
amongst genus and species-exclusive (young) genes compared to broadly
conserved (ancient) genes. Further, young genes exhibited lower expression, less
influence on viability, and accumulated nonsynonymous substitutions more frequently
than ancient genes. These findings demonstrate that young genes are enriched for
stress-responsiveness, may adapt rapidly, and therefore, may be important targets
for improving industrial stress tolerances.
Read in full at bioRxiv.
This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.