Sperm DNA damage causes genomic instability in early embryonic development

Sjors Middelkamp, Helena T.A. van Tol, Diana C.J. Spierings, Sander Boymans, Victor Guryev, Bernard A.J. Roelen, Peter M. Lansdorp, Edwin Cuppen, Ewart W. Kuijk

Jul 24, 2019
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Received Date: 15th July 19

Genomic instability is common in early embryo development, but the underlying causes are largely unknown. Here we examined the consequences of sperm DNA damage on the embryonic genome by single-cell genome sequencing of individual blastomeres from bovine embryos produced with sperm damaged by radiation. Sperm DNA damage caused fragmentation of chromosomes and segregation errors such as heterogoneic cell divisions yielding a broad spectrum of genomic aberrations that are similar to those frequently found in human embryos. The mosaic aneuploidies, mixoploidy, uniparental disomies and de novo structural variation induced upon sperm DNA damage may compromise health and lead to rare genomic disorders when embryos escape developmental arrest.

Read in full at bioRxiv.

This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

Nature Communications

Nature Research, Springer Nature