Quantitative Cellular-Resolution Map of the Oxytocin Receptor in Postnatally Developing Mouse Brains

Kyra T. Newmaster, Zachary T. Nolan, Uree Chon, Daniel J. Vanselow, Abigael R. Weit, Manal Tabbaa, Shizu Hidema, Katsuhiko Nishimori, Elizabeth A.D. Hammock, Yongsoo Kim

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Aug 22, 2019
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Received Date: 13th August 19

Oxytocin receptor (OTR) plays critical roles in social behavior development. Despite its significance, brain-wide quantitative understanding of OTR expression remains limited in postnatally developing brains. Here, we developed postnatal 3D template brains to register whole brain images with cellular resolution to systematically quantify OTR cell densities. We utilized fluorescent reporter mice (OTRvenus/+) and found that cortical regions showed temporally and spatially heterogeneous patterns with transient postnatal OTR expression without cell death. Cortical OTR cells were largely not GABAergic neurons with the exception of cells in layer 6b. Subcortical regions showed similar temporal regulation except the hypothalamus. Moreover, our unbiased approach identified two hypothalamic nuclei with sexually dimorphic OTR expression. Lastly, we created a website to visualize our high-resolution imaging data. In summary, our research establishes a developmental quantitative brain mapping method and provides a comprehensive resource for postnatal OTR expression in the mouse brain.

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This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

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