Local auxin biosynthesis promotes stem cell differentiation and organogenesis in Arabidopsis shoot apex

Shalini Yadav, Harish Kumar and Ram Kishor Yadav

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Nov 08, 2019
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Received Date: 4th November 19

One of the striking aspects of stem cells is their ability to self-renew to maintain the adequate pool over extended periods and differentiate into distinct cell types on demand to sustain the tissue growth and repair by supplying new cells. Arabidopsis plant harbors pluripotent stem cells in the central zone (CZ) of the shoot apex. The daughters of these cells upon cell division move towards the peripheral zone (PZ). At the flanks of the meristem, they form lateral organs. Cells that get displaced underneath CZ become part of the rib-meristem (RM). RM gives rise to stem tissue and vascular cell types in higher plants. Despite the three decades of genetic research work, our understanding of how stem cells differentiate into PZ and RM cell types is inadequate. Here, we show that locally produced auxin in combination with transport is essential for stem cell differentiation and organogenesis in the shoot apex. WUSCHEL, a homeodomain transcription factor, negatively regulates auxin biosynthesis in the stem cell niche to maintain the long-term pluripotency of shoot stem cells. Our findings reveal the role of local auxin biosynthesis in stem cell differentiation and how WUSCHEL regulates auxin signaling to promote stem cell fate in the shoot apex.

Read in full at bioRxiv.

This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

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