Growth on human milk oligosaccharides and co-growth on mucin via conserved pathways in early-colonizing and prevalent butyrate producing human gut Clostridiales

Michael Jakob Pichler, Chihaya Yamada, Bashar Shuoker, Camila Alvarez-Silva, Aina Gotoh, Maria Louise Leth, Erwin Schoof, Toshihiko Katoh, Mikiyasu Sakanaka, Takane Katayama, Chunsheng Jin, Niclas G Karlsson, Manimozhiyan Arumugam, Shinya Fushinobu, Maher Abou Hachem

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Received Date: 7th April 20

The early life human gut microbiota exerts life-long health effects on the host, but the mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of Clostridiales from the Roseburia-Eubacterium group, associated with protection from colorectal cancer, immune- and metabolic disorders is enigmatic. Here, we describe catabolic pathways that support the growth of Roseburia and Eubacterium members on distinct human milk oligosaccharides (HMOs). The HMO pathways, which include enzymes with a previously unknown structural fold and specificity, were upregulated together with additional glycan-utilization loci during growth on selected HMOs and in co-cultures with Akkermansia muciniphila on mucin, suggesting an additional role in enabling cross-feeding and access to mucin O-glycans. Analyses of 4599 Roseburia genomes underscored the preponderance and diversity of the HMO utilization loci within the genus. The catabolism of HMOs by butyrate-producing Clostridiales may contribute to the competitiveness of this group during the weaning-triggered maturation of the microbiota.

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This is an abstract of a preprint hosted on an independent third party site. It has not been peer reviewed but is currently under consideration at Nature Communications.

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